董健, 赵法兰, 徐北霜
Abstract
目的建立快捷、易行、准确的血清硒测定方法.方法利用氯酸在130℃恒温下消化样品,以流动注射氢化物原子吸收法测定血清硒.结果方法的线性范围为0～10 μg/L,检出限为0.48 μg/L,平均回收率为96.5%,合并变异系数为6.2%.结论测定方法具有灵敏度高、重现性好、结果准确、操作简单、一次测定样品量大等优点,很适合硒缺乏病病区调查研究及临床硒相关疾病诊治的需要.
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目的建立快捷、易行、准确的血清硒测定方法.方法利用氯酸在130℃恒温下消化样品,以流动注射氢化物原子吸收法测定血清硒.结果方法的线性范围为0～10 μg/L,检出限为0.48 μg/L,平均回收率为96.5%,合并变异系数为6.2%.结论测定方法具有灵敏度高、重现性好、结果准确、操作简单、一次测定样品量大等优点,很适合硒缺乏病病区调查研究及临床硒相关疾病诊治的需要.
Key concepts: Selenium, Atomic absorption spectroscopy, Hydride, Chemistry, Analytical Chemistry (journal), Digestion (alchemy), Constant (computer programming), Chromatography