ScholarLens Back to search
2011•Guoji yiyao weisheng daobaoRequires access

Determination of glycyrrhe tinic acid in Weikangling capsules by high-performance liquid chromatography

Su-hong Dai, Yang Ji-de Ou, Lixia Chen, Juan He

Open publisher page 0 citations

Abstract

Objective To establish a method for determinating the content of glycyrrhe tinic acid in Weikangling capsules by high-performance liquid chromatography ( HPLC ). HPLC was performed with SHIMADZU VP ODS( 4.6mm×250 mm, 5μtm ) as an analytical column and acetonitrile -3% acetic acid ( 70:30 ) as a mobile phase. The flow rate was 1.0 ml.min-1, the detection wavelength was 249nm; and the injection volume was 10μtl. Results The calibration curve of glycyrrhe tinic acid was linear within 10.70 - 214.08 μg.mL-1, with linear relationship Y = 1.53× 104X - 1.48× 102 ( r = 0.999 9 ). The average recovery of paeoniflorin was99.1%; RSD=1.1% (n=5).Conclusions This method is simple, accurate, and suitable for the determination of Weikangling capsules. Key words: Weikangling capsule;  Glycyrrhe tinic acid;  HPLC

About this research paper

What this paper is about

Objective To establish a method for determinating the content of glycyrrhe tinic acid in Weikangling capsules by high-performance liquid chromatography ( HPLC ). HPLC was performed with SHIMADZU VP ODS( 4.6mm×250 mm, 5μtm ) as an analytical column and acetonitrile -3% acetic acid ( 70:30 ) as a mobile phase. The flow rate was 1.0 ml.min-1, the detection wavelength was 249nm; and the injection volume was 10μtl. Results The calibration curve of glycyrrhe tinic acid was linear within 10.70 - 214.08 μg.mL-1, with linear relationship Y = 1.53× 104X - 1.48× 102 ( r = 0.999 9 ). The average recovery of paeoniflorin was99.1%; RSD=1.1% (n=5).Conclusions This method is simple, accurate, and suitable for the determination of Weikangling capsules. Key words: Weikangling capsule;  Glycyrrhe tinic acid;  HPLC

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To establish a method for determinating the content of glycyrrhe tinic acid in Weikangling capsules by high-performance liquid chromatography ( HPLC ). HPLC was performed with SHIMADZU VP ODS( 4.6mm×250 mm, 5μtm ) as an analytical column and acetonitrile -3% acetic acid ( 70:30 ) as a mobile phase. The flow rate was 1.0 ml.min-1, the detection wavelength was 249nm; and the injection volume was 10μtl. Results The calibration curve of glycyrrhe tinic acid was linear within 10.70 - 214.08 μg.mL-1, with linear relationship Y = 1.53× 104X - 1.48× 102 ( r = 0.999 9 ). The average recovery of paeoniflorin was99.1%; RSD=1.1% (n=5).Conclusions This method is simple, accurate, and suitable for the determination of Weikangling capsules. Key words: Weikangling capsule;  Glycyrrhe tinic acid;  HPLC

Key concepts: Chromatography, High-performance liquid chromatography, Paeoniflorin, Calibration curve, Chemistry, Capsule, Acetic acid, Acetonitrile

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of glycyrrhe tinic acid in Weikangling capsules by high-performance liquid chromatography — Research Paper | ScholarLens