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2007•Traditional Chinese MedicineRequires access

Determination of Paeoniflorn in naoxueshuan Capsules by HPLC

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Abstract

Objective To establish a RP-HPLC method for quantitative determination of Paeoniflorn in naoxueshuan Capsules.Methods The separation was performed on Dikma C18 column(4.6 mm×150 mm,5μm) with mobile phase of Acetonitrile and Water(14:86) at a flow rate of 1.0 mL/min.The UV detection wavelength was 230 nm.Results The linear range of Paeoniflorn was 0.156～0.364 μg,r=0.9999.The mean recovery was 98.32 %,RSD 0.83 %(n=5).Conclusion This method is simple,accurate,sensitive and with good reproducibility.

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Objective To establish a RP-HPLC method for quantitative determination of Paeoniflorn in naoxueshuan Capsules.Methods The separation was performed on Dikma C18 column(4.6 mm×150 mm,5μm) with mobile phase of Acetonitrile and Water(14:86) at a flow rate of 1.0 mL/min.The UV detection wavelength was 230 nm.Results The linear range of Paeoniflorn was 0.156～0.364 μg,r=0.9999.The mean recovery was 98.32 %,RSD 0.83 %(n=5).Conclusion This method is simple,accurate,sensitive and with good reproducibility.

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Available abstract

Objective To establish a RP-HPLC method for quantitative determination of Paeoniflorn in naoxueshuan Capsules.Methods The separation was performed on Dikma C18 column(4.6 mm×150 mm,5μm) with mobile phase of Acetonitrile and Water(14:86) at a flow rate of 1.0 mL/min.The UV detection wavelength was 230 nm.Results The linear range of Paeoniflorn was 0.156～0.364 μg,r=0.9999.The mean recovery was 98.32 %,RSD 0.83 %(n=5).Conclusion This method is simple,accurate,sensitive and with good reproducibility.

Key concepts: Chromatography, Reproducibility, High-performance liquid chromatography, Linear range, Acetonitrile, Linear relationship, Chemistry, Analytical Chemistry (journal)

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