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Content Determination of Ferullic Acid in Lingqi Juangan Capsules by HPLC

Ye Yun

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Abstract

OBJECTIVE:To establish a method for the content determination of ferullic acid in Lingqi juangan capsules. METHODS:HPLC method was adopted. The determination was performed on Phenomenex C18(250 mm×4.6 mm,5 μm) column with mobile phase consisted of acetonitrile-1% glacial acetic acid(32:68) at the flow rate of 1.0 mL·min-1. The detection wavelength was set at 320 nm and the column temperature was at 30 ℃. RESULTS:The linear range of ferullic acid were 9.84～98.40 μg·mL-1(r=0.999 4) with mean recoveries of 99.12%(RSD=0.28%,n=9) . CONCLUSION:The established method is simple,easy,precise and reproducible with good stability,and it is suitable for the content determination of ferullic acid in Lingqi juangan capsules.

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OBJECTIVE:To establish a method for the content determination of ferullic acid in Lingqi juangan capsules. METHODS:HPLC method was adopted. The determination was performed on Phenomenex C18(250 mm×4.6 mm,5 μm) column with mobile phase consisted of acetonitrile-1% glacial acetic acid(32:68) at the flow rate of 1.0 mL·min-1. The detection wavelength was set at 320 nm and the column temperature was at 30 ℃. RESULTS:The linear range of ferullic acid were 9.84～98.40 μg·mL-1(r=0.999 4) with mean recoveries of 99.12%(RSD=0.28%,n=9) . CONCLUSION:The established method is simple,easy,precise and reproducible with good stability,and it is suitable for the content determination of ferullic acid in Lingqi juangan capsules.

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Available abstract

OBJECTIVE:To establish a method for the content determination of ferullic acid in Lingqi juangan capsules. METHODS:HPLC method was adopted. The determination was performed on Phenomenex C18(250 mm×4.6 mm,5 μm) column with mobile phase consisted of acetonitrile-1% glacial acetic acid(32:68) at the flow rate of 1.0 mL·min-1. The detection wavelength was set at 320 nm and the column temperature was at 30 ℃. RESULTS:The linear range of ferullic acid were 9.84～98.40 μg·mL-1(r=0.999 4) with mean recoveries of 99.12%(RSD=0.28%,n=9) . CONCLUSION:The established method is simple,easy,precise and reproducible with good stability,and it is suitable for the content determination of ferullic acid in Lingqi juangan capsules.

Key concepts: Chromatography, Acetic acid, Content determination, High-performance liquid chromatography, Chemistry, Linear range, Acetonitrile, Detection limit

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