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Determination of Caffeotannic Acid in Jitongning Capsules by HPLC

Yonghui Sun

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Abstract

Objective An assay method was established for the determination of caffeotannic acid in Jitongning capsules by HPLC. Methods Kromasil C18 analytical column (200 mm×4.6 mm, 5 μm). Mobile phase was aceronitrile-0.4% H3PO4 solution (12∶88). Flow rate was 0.8 mL/min. The detecting wavelength was at 327 nm. Results The linear range of caffeotannic acid was 0.12～0.6 μg (r =0.9996). The average recovery was 100.0%. RSD=1.82%. Conclusion This method was proved to be simple, sensitive and reliable.

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What this paper is about

Objective An assay method was established for the determination of caffeotannic acid in Jitongning capsules by HPLC. Methods Kromasil C18 analytical column (200 mm×4.6 mm, 5 μm). Mobile phase was aceronitrile-0.4% H3PO4 solution (12∶88). Flow rate was 0.8 mL/min. The detecting wavelength was at 327 nm. Results The linear range of caffeotannic acid was 0.12～0.6 μg (r =0.9996). The average recovery was 100.0%. RSD=1.82%. Conclusion This method was proved to be simple, sensitive and reliable.

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Available abstract

Objective An assay method was established for the determination of caffeotannic acid in Jitongning capsules by HPLC. Methods Kromasil C18 analytical column (200 mm×4.6 mm, 5 μm). Mobile phase was aceronitrile-0.4% H3PO4 solution (12∶88). Flow rate was 0.8 mL/min. The detecting wavelength was at 327 nm. Results The linear range of caffeotannic acid was 0.12～0.6 μg (r =0.9996). The average recovery was 100.0%. RSD=1.82%. Conclusion This method was proved to be simple, sensitive and reliable.

Key concepts: Chromatography, High-performance liquid chromatography, Chemistry, Linear range, Recovery rate, Linear relationship, Content determination, Detection limit

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