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Determination of atropine sulfate and related substances in Atropine Sulfate Eye Drops by HPLC.

Bai RuoWan, Zhu JianDong, Pan JiFei, Wenfang Han, Li-Fen Wu

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Abstract

Objective To establish a HPLC method for the determination of atropine sulfate and related substances in Atropine Sulfate Eye Drops. Methods A C18 column was used and the mobile phase was phosphate buffer-methanol (85:15). The detection wavelength was 206 nm. Results A linear plot of peak area versus concentration of atropine sulfate was obtained in the range of 25.98～519.5 μg·mL-1 (r=0.999 99). The limit of detection was 8.25 ng. The average recovery was 99.71% with RSD of 0.30%. Conclusion With good accuracy and precision, this method can be used for the determination of atropine sulfate and related substances in Atropine Sulfate Eye Drops.

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Objective To establish a HPLC method for the determination of atropine sulfate and related substances in Atropine Sulfate Eye Drops. Methods A C18 column was used and the mobile phase was phosphate buffer-methanol (85:15). The detection wavelength was 206 nm. Results A linear plot of peak area versus concentration of atropine sulfate was obtained in the range of 25.98～519.5 μg·mL-1 (r=0.999 99). The limit of detection was 8.25 ng. The average recovery was 99.71% with RSD of 0.30%. Conclusion With good accuracy and precision, this method can be used for the determination of atropine sulfate and related substances in Atropine Sulfate Eye Drops.

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Available abstract

Objective To establish a HPLC method for the determination of atropine sulfate and related substances in Atropine Sulfate Eye Drops. Methods A C18 column was used and the mobile phase was phosphate buffer-methanol (85:15). The detection wavelength was 206 nm. Results A linear plot of peak area versus concentration of atropine sulfate was obtained in the range of 25.98～519.5 μg·mL-1 (r=0.999 99). The limit of detection was 8.25 ng. The average recovery was 99.71% with RSD of 0.30%. Conclusion With good accuracy and precision, this method can be used for the determination of atropine sulfate and related substances in Atropine Sulfate Eye Drops.

Key concepts: Atropine sulfate, Atropine, Chromatography, Chemistry, High-performance liquid chromatography, Sulfate, Detection limit, Anesthesia

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Determination of atropine sulfate and related substances in Atropine Sulfate Eye Drops by HPLC. — Research Paper | ScholarLens