2010•Chronic Pathematology JournalRequires access
Determination of the Contents of Atropine Sulfate Injection by HPLC
Deng Xue-yin
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Abstract
Objective To develop a HPLC internal standard method for determination of Atropine Sulfate Injection.Methods The C18 column (4.6 mm× 250 mm,5 μm)was used,acetonitrile-water-triethylamine (25:75:0.1,V /V) was used as mobile phase,flow rate:1.0 ml/min,The UV detection wavelength is 210 nm,Phenacetin Was selected as internal standard.Results The inearity was obtained from 40 to 200 μg/ml of Atropine Sulfate Injection(r =0.997 8,The average recoveries for Atropine Sulfate were 101.2%,97.6% and 102.3%,The RSD were less than 5%).Conclusion The method is simple,accurate and reliable and can be used for quality control of atropine sulfate injection.