2010•Agricultural Science and Technology HunanRequires access
Isolation and identification of a taxol-producing endophytic fungus identified from Taxus media.
Li TongQing, Zhijian Zhang, Peng Zhang, Chunlan Wang, Bo Liu, Tingting Liu, Fu ChunHua, Longjiang Yu
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Abstract
[Objective] The aim was to isolate and identify a taxol-producing endophytic fungus from Taxus media. [Method] 32 strains of endophytic fungi were identified form the inner bark of T. media,and their fermentation products were detected by high performance liquid chromatography (HPLC). [Result] Through the screening,a strain of taxol-producing endophytic fungi M57 was obtained,which could produce 45-50 μg/L of taxol,and M57 was defined as Rhizopus sp. through morphological observation and 18S rDNA sequence analysis. [Conclusion] The finding of Rhizopus sp. M57 provided a promising strain for producing taxol with taxol-producing fungi fermentation process.