2012•Hubei nongye kexueRequires access

Isolation and Identification of A Taxol-producing Endophytic Fungus O60B1

GU Yu-cheng

Abstract

A taxol-producing endophytic fungus O60B1 was isolated from the inner bark of Taxus x media.It could produce 2.5~3.0 μg taxol per one gram dry weight of mycelium according to determination of high performance liquid chromatography(HPLC) and mass spectrometry(MS).The fungus O60B1 was defined as Mucor sp.through morphological observation and 18 S rDNA sequence analysis.

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Key concepts: Mycelium, Fungus, Bark (sound), Plant use of endophytic fungi in defense, Taxus, Isolation (microbiology), Biology, High-performance liquid chromatography