Study on Endophytic Fungi Producing Taxol Isolated from Taxus Yunnanensis
Yang Dongliang
Abstract
Yang Dongliang
Abstract
More than 200 strains of endophytic fungi were isolated by aseptic techniques from the Taxus Yunnanensis in Yunnan Province. They were cultured in the liquid potato medium and indentified by means of the thin layer chromatography (TLC) and HPLC. The result showed one strain of fungus which was signed 12.3.2 could produce taxol in vitro,according to its morphology, which belonged to penicillium.. Using microbe (especially the fungi) to produce Taxol is considered to be a new way to obtain Taxol.So this strain is worthy to be further studied.
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More than 200 strains of endophytic fungi were isolated by aseptic techniques from the Taxus Yunnanensis in Yunnan Province. They were cultured in the liquid potato medium and indentified by means of the thin layer chromatography (TLC) and HPLC. The result showed one strain of fungus which was signed 12.3.2 could produce taxol in vitro,according to its morphology, which belonged to penicillium.. Using microbe (especially the fungi) to produce Taxol is considered to be a new way to obtain Taxol.So this strain is worthy to be further studied.
Key concepts: Taxus, Plant use of endophytic fungi in defense, Biology, Thin-layer chromatography, Botany, Fungus, Penicillium, Strain (injury)