Determination of Four Effective Constituents in Liuwei Dihuang Pills by HPLC

An HPLC method was established for the simultaneous determination of paeonol,loganin,morroniside and paeoniflorin in Liuwei Dihuang pills.A C18 column was used with the mobile phase of acetonitrile-0.1% phosphoric acid for gradient elution at the detection wavelength of 238 nm.The calibration curves of the four constituents were linear in the concentration ranges of 0.5-20,0.25-10,0.5-20 and 0.15-6 μg/ml,respectively.The recoveries were 98.10%-105.44%.