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Simultaneous determination of three constituents in Liuwei Dihuang pill via HPLC

Wei Yu-hu

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Abstract

Objective An HPLC method was established for determining three constituents(Loganin,paeoniflorin and paeonol) in Liuwei Dihuang pill.Methods HPLC assay was carried out using a VP-ODS column.The mobile phase was consisted of acetonitrile-water gradient system.The flow rate was 0.8ml/min,and wavelength was set at 230nm.The column temperature was 25℃.Results The calibration curve was linear over the concentration range of 1.56~50.00μg/ml for loganin,0.47~15.00 μg/ml for paeoniflorin,and 6.25~200.00μg/ml for paeonol,respectively.The correlation coefficient of all curves were better than 0.9992.The average recoveries of three analytes are more than 99.17%,and the RSD value of precision was less than 2.54%.Conclusion The developed HPLC method could be used for the quality control of Liuwei Dihuang pill.

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What this paper is about

Objective An HPLC method was established for determining three constituents(Loganin,paeoniflorin and paeonol) in Liuwei Dihuang pill.Methods HPLC assay was carried out using a VP-ODS column.The mobile phase was consisted of acetonitrile-water gradient system.The flow rate was 0.8ml/min,and wavelength was set at 230nm.The column temperature was 25℃.Results The calibration curve was linear over the concentration range of 1.56~50.00μg/ml for loganin,0.47~15.00 μg/ml for paeoniflorin,and 6.25~200.00μg/ml for paeonol,respectively.The correlation coefficient of all curves were better than 0.9992.The average recoveries of three analytes are more than 99.17%,and the RSD value of precision was less than 2.54%.Conclusion The developed HPLC method could be used for the quality control of Liuwei Dihuang pill.

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Available abstract

Objective An HPLC method was established for determining three constituents(Loganin,paeoniflorin and paeonol) in Liuwei Dihuang pill.Methods HPLC assay was carried out using a VP-ODS column.The mobile phase was consisted of acetonitrile-water gradient system.The flow rate was 0.8ml/min,and wavelength was set at 230nm.The column temperature was 25℃.Results The calibration curve was linear over the concentration range of 1.56~50.00μg/ml for loganin,0.47~15.00 μg/ml for paeoniflorin,and 6.25~200.00μg/ml for paeonol,respectively.The correlation coefficient of all curves were better than 0.9992.The average recoveries of three analytes are more than 99.17%,and the RSD value of precision was less than 2.54%.Conclusion The developed HPLC method could be used for the quality control of Liuwei Dihuang pill.

Key concepts: Loganin, Paeoniflorin, Paeonol, Chromatography, High-performance liquid chromatography, Calibration curve, Correlation coefficient, Medicine

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