Simultaneous Determination of Three Constituents in Liuwei Dihuang Capusule by RP-HPLC

[Objective] To establish RP-HPLC method for simultaneous determining paeoniflorin,loganin and paeonol in Liuwei Dihuang Capsule.[Method]RP-HPLC assay was carried out using a Waters ODS C18(4.6 × 250,5) column.The mobile phase was consisted of methanol-0.1% phosphoric acid in gradient elution.The flow rate was 1.0 ml/min.The UV detection wavelength was set at 230 for paeoniflorin and loganin,and 274 for paeonol.The column temperature was 40 ℃.[Result] The linear ranges of paeoniflorin,loganin and paeonol were0.066 5-1.663 2(r = 0.999 4),0.107 9-2.697 7(r = 0.9998),0.122 4-3.060 0(r = 0.999 9),respectively.The average recoveries of paeoniflorin,loganin and paeonol were 97.63%(RSD was 0.82%),97.66%(RSD was 0.74%),and 96.22%(RSD was1.21%),respectively.[Conclusion] The method is simple,accurate and reproducible.It can be used for the quality control of Liuwei Dihuang Capsule.