Determination of Liuwei Dihuang Pill(concentrated pill)

Objective To establish an HPLC for determination of paeonol and loganin in Liuwei Dihang Pills(concentrated pills).Methods Paeonol :HPLC column was C18.The mobile phase was methanol-water(6∶4) and the flow rate was 1.0 ml·min-1.The column temperature was at 30℃ and the detection wavelength was at 274 nm.Loganin:HPLC column was C18.The mobile phase was acetonitrile-water(15∶85)and the flow rate was 1.0 ml·min-1.The column temperature was at 25℃ and the detection wavelength was at 236 nm.Results The linear range was from 54.1ng to 270.5 ng(r=0.999 6) for paeonol and from 0.223 μg to 4.46 μg(r=0.999 6) for loganin.Conclusion The method is convenient and reproducible for determination of paeonol and loganin in Liuwei Dihuang Pills(concentrated pills)