Purification of total saponins from Asparagus officinalis by macroporous resin

Objective To investigate the purification of saponins from Asparagus officinalis by macroporous resin.Methods Macroporous resin was chosen with static and dynamic adsorption and desorption experiments to optimize the purification parameters.Results S-8 macroporous resin was found to have good adsorption and desorption effects and show an excellent decolor effect.The optimum purification conditions were 1 mg/mL of saponins eluted by 75% ethanol at pH value 7 with a volume of 10 mL/g,and the loaded amount was 60 mL/g.The purity of total saponons increased to 50.15% after the purification,and the decoloration rate maintained 92.23%.Conclusion S-8 is an ideal resin with the best enrichment for separating and purifying total saponins from A.officinalis.