Optimization of Separation and Purification Process for Total Saponins from Bolbostemma paniculatum with Macroporous Resin

Objective:To optimize separation and purification process parameters of total saponins from Bolbostemma paniculatum by macroporous resin.Method: With absorption and desorption rate of total saponins as indexes,separation and purification performance of macroporous resin on total saponins from B.paniculatum was compared by static and dynamic test,optimum resin was selected and its purification technology was optimized.Result: HPD-750 macroporous resin showed the best property for purification of total saponins.its optimal process conditions were:diameter-height ratio 1∶5,the concentration of sample solution 1.6 g·mL-1,maximum sample volume 13 mL,eluted by 1 BV water and 8 BV 95% ethanol,flow rate of adsorption and elution 1 mL·min-1,eluent were collected,ethanol was recovered,under these conditions,the content of total saponins was 63.53% by UV.Conclusion: This optimized process was simple and feasible to separate and purify total saponins from B.paniculatum effectively.