Optimization of Purification Technology of Total Saponins from Allium sativum with Macroporous Resins

Objective: To optimize purification technology of total saponins from Allium sativum with macroporous resin.Method: With transfer rate of total saponins as index,single factor tests were adopted to optimize purification process parameters,such as type of macroporous resin,adsorption capacity of resin(adsorption capacity,the concentration of sample solution,pH and adsorption speed) and elution performance(the concentration,flow rate and volume of eluent).Result: Optimized purification technology was as following: selected AB-8 macroporous resin,ratio of crude drug-resin 1.3 ∶1,the concentration of sample solution 0.8 g.mL-1,pH 7,adsorption speed 2 BV.h-1,removed impurities with 30% ethanol,eluted with 5 BV of 95% ethanol,elution speed 3 BV.h-1.Under these conditions,purity of total saponins was over 65%.Conclusion: AB-8 macroporous resin could be adopted to purify total saponins from A.sativum,this optimized purification technology was stable and feasible.