Purification of saponins from Asparagus officinalis by macroporous adsorption resin

An optimal macroporous adsorption resin and its optimal technological parameters for purification of saponins from extracts of Asparagus officinalis were investigated in the present study.Through static method,comparing the saponin purification result of 8 resins,HPD-100 resin was chosen due to its excellent adsorption and desorption performance.Optimal conditions were primarily confirmed as follows:sample concentration 15mg/mL with loading amount of 150mL at a flow rate of 1mL/min,washing with 200mL 80% ethanol at a flow rate of 1mL/min.Sample was purified by HPD-100 resin according to these technology parameters and the content of saponins reached 35.49%.Then the primary purification product was purified in accordance with the above process,and the content of saponins could be higher than 50% and complied with the standards of class V of new Chinese drug.These datas demonstrated the excellence of HPD-100 resin in purifying saponins from A.officinalis and the advantages of the developed method for industrial application,like simple operation,safety and low cost.