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Callus culture of Cinnamomum tenuipilum

Ligang Zhou, Biqiang Cheng, Xuejian Yu, Wang Junjian, Chong‐Ren Yang

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Abstract

Galli were induced from the leaves explants of a chemical type of Cinnamonmum tenuipilum Kosterm in which the main aromatic component was geraniol. It was optimal for the callus growth by using MS medium in which IAA 2mg/L and KT 0.1mg/L were added into it. The optimum pH value in the medium was about 5-6, and optimum temperature was about 25℃. Light inhibited callus growth. Preliminary identification of geraniol in the callus was carried out by gas chromatography. The results demonstrated that callus was capable of synthesizing geraniiol the same as that of the original plant.

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What this paper is about

Galli were induced from the leaves explants of a chemical type of Cinnamonmum tenuipilum Kosterm in which the main aromatic component was geraniol. It was optimal for the callus growth by using MS medium in which IAA 2mg/L and KT 0.1mg/L were added into it. The optimum pH value in the medium was about 5-6, and optimum temperature was about 25℃. Light inhibited callus growth. Preliminary identification of geraniol in the callus was carried out by gas chromatography. The results demonstrated that callus was capable of synthesizing geraniiol the same as that of the original plant.

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Available abstract

Galli were induced from the leaves explants of a chemical type of Cinnamonmum tenuipilum Kosterm in which the main aromatic component was geraniol. It was optimal for the callus growth by using MS medium in which IAA 2mg/L and KT 0.1mg/L were added into it. The optimum pH value in the medium was about 5-6, and optimum temperature was about 25℃. Light inhibited callus growth. Preliminary identification of geraniol in the callus was carried out by gas chromatography. The results demonstrated that callus was capable of synthesizing geraniiol the same as that of the original plant.

Key concepts: Callus, Geraniol, Explant culture, Chemistry, Botany, Tissue culture, Horticulture, Chromatography

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