Studies on tissue culture of cistanche salsa(C. A. Mey.) G. beck

To probe into the active ingredient production, the tissue culture is studied with the stem segment and flower of Cistanche salsa(C.A.Mey.) G.Beck. The result shows that when the medium contains 2,4D,the shapes of callus inducted from the stem and ovary explants are block and grain respectively and the color is white. But when the medium contains KT, the callus appears as grain and kelly. The findings show that the MT mediums containing KT,NAA have the best effects on the callus induction and growth. The callus is made to contact with the medium to culture for a certain time before they are separated from the mother explant for cotiune culture, as is good for the growth of the callus.