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Determination of Catechin Content in RHIZOMA BERGENIAE SCOPULOSAE by HPLC

Feng YongHui

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Abstract

[Objective] The aim was to establish a determination method for the catechin content of RHIZOMA BERGENIAE SCOPULOSAE by HPLC.[Method] Ultrasonic-assisted extraction was adopted and HPLC was used for the determination.The chromatographic conditions were:Kromasil-C18(4.6 mm×150 mm,5 μm) as the column,methanol-water-glacial acetic acid(V/V/V,15:84:1) as the mobile phase,1.0 ml/min flow rate,and 276 nm detection wavelength.[Result] The linear range of catechins was between 0.624 and 3.12 μg/ml(r=0.999 3);the average recovery was 100.05%,with RSD=1.73%(n=6).[Conclusion] This method was simple and accurate,and could be used for the determination of catechins in RHIZOMA BERGENIAE SCOPULOSAE.

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What this paper is about

[Objective] The aim was to establish a determination method for the catechin content of RHIZOMA BERGENIAE SCOPULOSAE by HPLC.[Method] Ultrasonic-assisted extraction was adopted and HPLC was used for the determination.The chromatographic conditions were:Kromasil-C18(4.6 mm×150 mm,5 μm) as the column,methanol-water-glacial acetic acid(V/V/V,15:84:1) as the mobile phase,1.0 ml/min flow rate,and 276 nm detection wavelength.[Result] The linear range of catechins was between 0.624 and 3.12 μg/ml(r=0.999 3);the average recovery was 100.05%,with RSD=1.73%(n=6).[Conclusion] This method was simple and accurate,and could be used for the determination of catechins in RHIZOMA BERGENIAE SCOPULOSAE.

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Available abstract

[Objective] The aim was to establish a determination method for the catechin content of RHIZOMA BERGENIAE SCOPULOSAE by HPLC.[Method] Ultrasonic-assisted extraction was adopted and HPLC was used for the determination.The chromatographic conditions were:Kromasil-C18(4.6 mm×150 mm,5 μm) as the column,methanol-water-glacial acetic acid(V/V/V,15:84:1) as the mobile phase,1.0 ml/min flow rate,and 276 nm detection wavelength.[Result] The linear range of catechins was between 0.624 and 3.12 μg/ml(r=0.999 3);the average recovery was 100.05%,with RSD=1.73%(n=6).[Conclusion] This method was simple and accurate,and could be used for the determination of catechins in RHIZOMA BERGENIAE SCOPULOSAE.

Key concepts: Chromatography, Chemistry, Catechin, High-performance liquid chromatography, Content determination, Acetic acid, Linear range, Extraction (chemistry)

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