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HPLC determination of bergeninum in two kinds of Rhizoma Rodgersiae Sambucifoliae

Yan Xiang-jie

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Abstract

Purpose To establish a method for determination of bergeninum in Rhizoma Rodgersiae Sambucifoliae by HPLC.Methods The HPCL system was conducted as follows:Hypersil C18 column(4.6 mm ×250 mm,5 μm),acetonitrile-water(10∶ 90)mixture as a mobile phase,detection wavelength at 274 nm,1.0 mL/min of flow rate and column temperature at 25 ℃.Results The linear range of bergeninum was 0.049 21-3.396 8 μg,r=0.999 8,the recovery was 99.8% and RSD=1.18(n=9).Conclusion The method is simple,accurate of higher sensitivity and reproducibe.This method can be used for the determination of bergeninum in Rhizoma Rodgersiae Sambucifoliae.

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Purpose To establish a method for determination of bergeninum in Rhizoma Rodgersiae Sambucifoliae by HPLC.Methods The HPCL system was conducted as follows:Hypersil C18 column(4.6 mm ×250 mm,5 μm),acetonitrile-water(10∶ 90)mixture as a mobile phase,detection wavelength at 274 nm,1.0 mL/min of flow rate and column temperature at 25 ℃.Results The linear range of bergeninum was 0.049 21-3.396 8 μg,r=0.999 8,the recovery was 99.8% and RSD=1.18(n=9).Conclusion The method is simple,accurate of higher sensitivity and reproducibe.This method can be used for the determination of bergeninum in Rhizoma Rodgersiae Sambucifoliae.

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Available abstract

Purpose To establish a method for determination of bergeninum in Rhizoma Rodgersiae Sambucifoliae by HPLC.Methods The HPCL system was conducted as follows:Hypersil C18 column(4.6 mm ×250 mm,5 μm),acetonitrile-water(10∶ 90)mixture as a mobile phase,detection wavelength at 274 nm,1.0 mL/min of flow rate and column temperature at 25 ℃.Results The linear range of bergeninum was 0.049 21-3.396 8 μg,r=0.999 8,the recovery was 99.8% and RSD=1.18(n=9).Conclusion The method is simple,accurate of higher sensitivity and reproducibe.This method can be used for the determination of bergeninum in Rhizoma Rodgersiae Sambucifoliae.

Key concepts: Chromatography, High-performance liquid chromatography, Chemistry, Linear range, Linear relationship, Acetonitrile, Content determination, Volumetric flow rate

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