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2011•Pharmaceutical Journal of Chinese People's Liberation ArmyRequires access

Determination of Catechin in Dahuang by High Performance Liquid Chromatography

Tan Sheng-jian

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Abstract

Objective To establish a method for determining catechin in Dahuang.Methods High performance liquid chromatography was used for the determination of the content of catechin in Dahuang.The chromatographic column was Prevail Select C18(150 mm ×4.6 mm,5 μm)and the column temperature was 35℃.The mobile phase consisted of acetonitrile-water-phosphoric acid(100:900:1).The flow rate was 1 ml/min,detected at 278 nm.Results The linear range of catechin was 22.18-221.8μg/ml,r=0.9999.The average recovery of catechin was 99.1% and RSD was 1.9%.Conclusion This method is simple,accurate and sensitive.It can be used for routine analysis of catechin in Dahuang.

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What this paper is about

Objective To establish a method for determining catechin in Dahuang.Methods High performance liquid chromatography was used for the determination of the content of catechin in Dahuang.The chromatographic column was Prevail Select C18(150 mm ×4.6 mm,5 μm)and the column temperature was 35℃.The mobile phase consisted of acetonitrile-water-phosphoric acid(100:900:1).The flow rate was 1 ml/min,detected at 278 nm.Results The linear range of catechin was 22.18-221.8μg/ml,r=0.9999.The average recovery of catechin was 99.1% and RSD was 1.9%.Conclusion This method is simple,accurate and sensitive.It can be used for routine analysis of catechin in Dahuang.

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Available abstract

Objective To establish a method for determining catechin in Dahuang.Methods High performance liquid chromatography was used for the determination of the content of catechin in Dahuang.The chromatographic column was Prevail Select C18(150 mm ×4.6 mm,5 μm)and the column temperature was 35℃.The mobile phase consisted of acetonitrile-water-phosphoric acid(100:900:1).The flow rate was 1 ml/min,detected at 278 nm.Results The linear range of catechin was 22.18-221.8μg/ml,r=0.9999.The average recovery of catechin was 99.1% and RSD was 1.9%.Conclusion This method is simple,accurate and sensitive.It can be used for routine analysis of catechin in Dahuang.

Key concepts: Catechin, Chromatography, Phosphoric acid, High-performance liquid chromatography, Acetonitrile, Chemistry, Linear range, Detection limit

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