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2013•Zhongguo shiyan fangjixue zazhiRequires access

Determination of Catechin in Eucommia ulmoids by RP-HPLC

LU Cheng-sheng

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Abstract

Objective:To establish a HPLC method for the determination of catechin in Eucommia ulmoides.Method: The orthogonal test was used to optimize the pretreatment process.RP-HPLC was adopted,on a column of Agilent HC-C18(4.6 mm×250 mm,5 μm),using the mobile phase of acetonitrile-0.4% phosphoric acid solution(13∶87),at a flow rate of 1.0 mL·min-1;the detection wavelength was set at 280 nm.Result: Catechin was linear in the range of 9.620-194.0 μg·mL-1(r=0.999 3).The average recovery of catechin was 97.38% with RSD 2.1%.Conclusion: The method is simple,rapid and reliable with good reproduciblity,which can be used for the content determination of catechin in E.ulmoides.

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Objective:To establish a HPLC method for the determination of catechin in Eucommia ulmoides.Method: The orthogonal test was used to optimize the pretreatment process.RP-HPLC was adopted,on a column of Agilent HC-C18(4.6 mm×250 mm,5 μm),using the mobile phase of acetonitrile-0.4% phosphoric acid solution(13∶87),at a flow rate of 1.0 mL·min-1;the detection wavelength was set at 280 nm.Result: Catechin was linear in the range of 9.620-194.0 μg·mL-1(r=0.999 3).The average recovery of catechin was 97.38% with RSD 2.1%.Conclusion: The method is simple,rapid and reliable with good reproduciblity,which can be used for the content determination of catechin in E.ulmoides.

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Available abstract

Objective:To establish a HPLC method for the determination of catechin in Eucommia ulmoides.Method: The orthogonal test was used to optimize the pretreatment process.RP-HPLC was adopted,on a column of Agilent HC-C18(4.6 mm×250 mm,5 μm),using the mobile phase of acetonitrile-0.4% phosphoric acid solution(13∶87),at a flow rate of 1.0 mL·min-1;the detection wavelength was set at 280 nm.Result: Catechin was linear in the range of 9.620-194.0 μg·mL-1(r=0.999 3).The average recovery of catechin was 97.38% with RSD 2.1%.Conclusion: The method is simple,rapid and reliable with good reproduciblity,which can be used for the content determination of catechin in E.ulmoides.

Key concepts: Catechin, Chromatography, Eucommia ulmoides, Chemistry, Phosphoric acid, High-performance liquid chromatography, Linear range, Polyphenol

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