The distribution and antibiotic susceptibility of producing extended-spectrum beta-lactamases(ESBLs)bacteria in Wuhan area in 2000

Objective To investigate the distribution and antibiotic susceptibility of producing extended spectrum beta-lactamases(ESBLs)bacteria.Methods API or VITEK AMS system were used to identify the bacteria;ESBLs were detected by NCCLS recommended methods and the susceptibilities to 14 kinds of antibiotics were tested by K B method.Results The incidences of ESBLs among 269 strains of Escherichia coli,202 strains of Klebsiella pneumoniae,149 strains of Enterobacter cloacae,67 strains of Citrobacter freundii and 43 strains of K.oxytoca found in Wuhan in 2000 were 40.9%,29.2%,20.1%,29.9%,7.7% and 23.3% respectively.All of the ESBLs producing E.coli and K.pneumoniae were susceptible to imipenem.The resistance rate of ESBLs producing E.coli to Amikacin was 22.4%,while these E.coli were resistant to other antibiotic at different levels.Conclusion The ESBLs producing bacteria mainly belong to Enterobacteriaceae E.coli,K.pneumoniae and C.freundii can produce higher level of ESBLs than others.The ESBLs produced by E.cloacae,K.oxytoca and E.cloacae are serious challenges for the control of infectious diseases.Imipenem is a good choice for the therapy of ESBLs producing bacteria.