2010•Unpublished venueRequires access

Determination of levofloxacin hydrochloride in different transfusion by HPLC

Wu Lei

Abstract

OBJECTIVE:To analyze and determine the content of levofloxacin hydrochloride in different transfusion by HPLC.METHODS:The HPLC system consisted of Eclipse XDB-C18(150mm×4.6mm,5 m),sodium hexanesulfonate solution-methanol(3:1) as a mobile phase,1 mL·min-1 of flow rate,detection wavelength at 293nm.RESULTS:The linear range of Levofloxacin hydrochloride was 0.55 g-2.75μg(r=0.9999),the recovery were 100.1%(RSD=0.62%,).CONCLUSION:The method is simple,sensitive,accurate and reproducible and it can be used for the quality control of Levofloxacin hydrochloride in different transfusion liquid.

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Key concepts: High-performance liquid chromatography, Levofloxacin, Chromatography, Chemistry, Hydrochloride, Recovery rate, Linear range, Detection limit