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HPLC determination of ethyl paraben in Levofloxacin Hydrochloride Eye Drops.

Wu LiFen, Bai RuoWan, Naicheng Li, Han BaoPing, Wenfang Han

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Abstract

Objective To establish an HPLC method for the determination of ethylparaben in Levofloxacin Hydrochloride Eye Drops.Methods An ODS column was used with 0.005 moL/L ammonium acetate(10 mL triethylamine in 1 000 mL solution adjusted to pH 5.0 with glacial acetic acid)-acetonitrile(50:50)as the mobile phase.The detection wavelength was 256 nm and the flow rate was 1.0 mL/min.The column temperature was kept at 35 ℃.Results The calibration curve of ethyl hydroxybenzoate was linear in the concentration range of 0.017 7-0.041 3 mg/mL(r= 0.999 97).The average recovery was 100.4 %(n = 9,RSD = 0.97 %).Conclusion The method is simple,rapid and accurate,and can be used for the quality control of Levofloxacin Hydrochloride Eye Drops.

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What this paper is about

Objective To establish an HPLC method for the determination of ethylparaben in Levofloxacin Hydrochloride Eye Drops.Methods An ODS column was used with 0.005 moL/L ammonium acetate(10 mL triethylamine in 1 000 mL solution adjusted to pH 5.0 with glacial acetic acid)-acetonitrile(50:50)as the mobile phase.The detection wavelength was 256 nm and the flow rate was 1.0 mL/min.The column temperature was kept at 35 ℃.Results The calibration curve of ethyl hydroxybenzoate was linear in the concentration range of 0.017 7-0.041 3 mg/mL(r= 0.999 97).The average recovery was 100.4 %(n = 9,RSD = 0.97 %).Conclusion The method is simple,rapid and accurate,and can be used for the quality control of Levofloxacin Hydrochloride Eye Drops.

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Available abstract

Objective To establish an HPLC method for the determination of ethylparaben in Levofloxacin Hydrochloride Eye Drops.Methods An ODS column was used with 0.005 moL/L ammonium acetate(10 mL triethylamine in 1 000 mL solution adjusted to pH 5.0 with glacial acetic acid)-acetonitrile(50:50)as the mobile phase.The detection wavelength was 256 nm and the flow rate was 1.0 mL/min.The column temperature was kept at 35 ℃.Results The calibration curve of ethyl hydroxybenzoate was linear in the concentration range of 0.017 7-0.041 3 mg/mL(r= 0.999 97).The average recovery was 100.4 %(n = 9,RSD = 0.97 %).Conclusion The method is simple,rapid and accurate,and can be used for the quality control of Levofloxacin Hydrochloride Eye Drops.

Key concepts: Chromatography, Paraben, Chemistry, Levofloxacin, High-performance liquid chromatography, Calibration curve, Triethylamine, Acetic acid

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HPLC determination of ethyl paraben in Levofloxacin Hydrochloride Eye Drops. — Research Paper | ScholarLens