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DETERMINATION OF LYSINE ASPIRIN IN PLASMA BY RP-HPLC

Zhao Kui

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Abstract

OBJECTIVE:To establish an HPLC method for the determination of lysine aspirin in plasma and to study the pharmacokinetics of its preparations.METHODS:Lysine aspirin in plasma was turned to salicylic acid by hydrolysis with sodium hydroxide and extracted with ethyl acetate and analyzed on a μBondapak ODS columm with phosphate buffer(pH 2.0 ) methanol (65∶35).UV detection wavelength was 305 nm and phenacetion was used as the internal standard.RESUITS:The method was linear within the range of 1～100 μg·ml -1 (r=0.9999)and the detection limit was 0.2 μg·ml -1 .The recovery was 104%～112%.Within run and between run precision (RSD) was 1.8%～ 4.0 % and 4.2%～6.8%,respectively.CONCLUSION:The method is simple and accurate and is very suitable for pharmacokinetics and bioavailability study of lysine aspirin preparations.

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What this paper is about

OBJECTIVE:To establish an HPLC method for the determination of lysine aspirin in plasma and to study the pharmacokinetics of its preparations.METHODS:Lysine aspirin in plasma was turned to salicylic acid by hydrolysis with sodium hydroxide and extracted with ethyl acetate and analyzed on a μBondapak ODS columm with phosphate buffer(pH 2.0 ) methanol (65∶35).UV detection wavelength was 305 nm and phenacetion was used as the internal standard.RESUITS:The method was linear within the range of 1～100 μg·ml -1 (r=0.9999)and the detection limit was 0.2 μg·ml -1 .The recovery was 104%～112%.Within run and between run precision (RSD) was 1.8%～ 4.0 % and 4.2%～6.8%,respectively.CONCLUSION:The method is simple and accurate and is very suitable for pharmacokinetics and bioavailability study of lysine aspirin preparations.

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Available abstract

OBJECTIVE:To establish an HPLC method for the determination of lysine aspirin in plasma and to study the pharmacokinetics of its preparations.METHODS:Lysine aspirin in plasma was turned to salicylic acid by hydrolysis with sodium hydroxide and extracted with ethyl acetate and analyzed on a μBondapak ODS columm with phosphate buffer(pH 2.0 ) methanol (65∶35).UV detection wavelength was 305 nm and phenacetion was used as the internal standard.RESUITS:The method was linear within the range of 1～100 μg·ml -1 (r=0.9999)and the detection limit was 0.2 μg·ml -1 .The recovery was 104%～112%.Within run and between run precision (RSD) was 1.8%～ 4.0 % and 4.2%～6.8%,respectively.CONCLUSION:The method is simple and accurate and is very suitable for pharmacokinetics and bioavailability study of lysine aspirin preparations.

Key concepts: Chromatography, Chemistry, Salicylic acid, Aspirin, Bioavailability, Sodium hydroxide, Sodium acetate, High-performance liquid chromatography

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