2000•Journal of Guangxi Agricultured and Biological ScienceRequires access

Determination of recibufogenin in Liushen injection by high performance liquid chromatography

Yao Pinjia

Abstract

A HPLC method for the determination of recibufogenin in Liushen injection was investigated. The chromatographic conditions had been revealed:ODS(6 0 mm×150 mm)column, mobile phase acetonitrilewater 55/45( V/V ),flow rate 0 8 mL/min, UV-detector(λ=299 nm). The average recovery was 96 95% and the relative standard deviation was 0 79%.

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Key concepts: Chromatography, High-performance liquid chromatography, Relative standard deviation, Volumetric flow rate, Standard deviation, Materials science, Recovery rate, Analytical Chemistry (journal)