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Content Determination of Viterxin in Xiaoyerong by HPLC

Hua Chen

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Abstract

Objective:To establish an HPLC method for the determination of Viterxin in Xiaoyerong.Methods LUNA ODS C18 BDS(250 mm×4.6 mm,5 μm)was used with acetonitrile-1%Acetic Acid(14∶86)as mobile phase.The flow rate was 1.0 ml·min-1.The detection wavelength was 340 nm.Results The linear range of Viterxin was 0.082 4~0.824 μg,r=0.999 7.The average recovery was 100.39%,RSD was 2.20%.Conclusion The method is simple,rapid and accurate.

About this research paper

What this paper is about

Objective:To establish an HPLC method for the determination of Viterxin in Xiaoyerong.Methods LUNA ODS C18 BDS(250 mm×4.6 mm,5 μm)was used with acetonitrile-1%Acetic Acid(14∶86)as mobile phase.The flow rate was 1.0 ml·min-1.The detection wavelength was 340 nm.Results The linear range of Viterxin was 0.082 4~0.824 μg,r=0.999 7.The average recovery was 100.39%,RSD was 2.20%.Conclusion The method is simple,rapid and accurate.

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Available abstract

Objective:To establish an HPLC method for the determination of Viterxin in Xiaoyerong.Methods LUNA ODS C18 BDS(250 mm×4.6 mm,5 μm)was used with acetonitrile-1%Acetic Acid(14∶86)as mobile phase.The flow rate was 1.0 ml·min-1.The detection wavelength was 340 nm.Results The linear range of Viterxin was 0.082 4~0.824 μg,r=0.999 7.The average recovery was 100.39%,RSD was 2.20%.Conclusion The method is simple,rapid and accurate.

Key concepts: Chromatography, High-performance liquid chromatography, Linear range, Acetic acid, Recovery rate, Chemistry, Acetonitrile, Linear relationship

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