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2007•Zhongguo zhongyiyao xinxi zazhiRequires access

HPLC Determination of Paeoniflorin in Huachanahuduji and Zhichadupeiji of Meihuadianshe Den

SU Fu-li

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Abstract

Objective To establish HPLC method for determination of Huachanshuduji and Zhichadupeiji in Chansu of Meihuadiansheoan.Metsod HPLC system includes ODS column,4.6mm×250mm,the mobile phase was acetonitrile-water(43.5:56.5),the flow rate was 1.0mL/min,and detection wavelength was 296 nm.The amount of injection was 20μL Results the calibration curves of Huachanshuduji and Zhichadupeiji were linear in the range of 5.31～63.75μg/mL and 6.00～72.00μg/mL(r=0.9998,0.9997). The average recoveries concentration added in samples were 99.45%,99.39%.The deviation was 1.32%, 0.55%(n=6).Conclusion The method is rapid,accurate and reliable.

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Objective To establish HPLC method for determination of Huachanshuduji and Zhichadupeiji in Chansu of Meihuadiansheoan.Metsod HPLC system includes ODS column,4.6mm×250mm,the mobile phase was acetonitrile-water(43.5:56.5),the flow rate was 1.0mL/min,and detection wavelength was 296 nm.The amount of injection was 20μL Results the calibration curves of Huachanshuduji and Zhichadupeiji were linear in the range of 5.31～63.75μg/mL and 6.00～72.00μg/mL(r=0.9998,0.9997). The average recoveries concentration added in samples were 99.45%,99.39%.The deviation was 1.32%, 0.55%(n=6).Conclusion The method is rapid,accurate and reliable.

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Available abstract

Objective To establish HPLC method for determination of Huachanshuduji and Zhichadupeiji in Chansu of Meihuadiansheoan.Metsod HPLC system includes ODS column,4.6mm×250mm,the mobile phase was acetonitrile-water(43.5:56.5),the flow rate was 1.0mL/min,and detection wavelength was 296 nm.The amount of injection was 20μL Results the calibration curves of Huachanshuduji and Zhichadupeiji were linear in the range of 5.31～63.75μg/mL and 6.00～72.00μg/mL(r=0.9998,0.9997). The average recoveries concentration added in samples were 99.45%,99.39%.The deviation was 1.32%, 0.55%(n=6).Conclusion The method is rapid,accurate and reliable.

Key concepts: Paeoniflorin, Chromatography, High-performance liquid chromatography, Calibration curve, Chemistry, Relative standard deviation, Acetonitrile, Linear relationship

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HPLC Determination of Paeoniflorin in Huachanahuduji and Zhichadupeiji of Meihuadianshe Den — Research Paper | ScholarLens