Purification of effective components from Smilacina japonica by macroporous adsorption resin

Objective To optimize the parameters of purification process of total saponins and flavonoids from Smilacina japonica A.Gray by macroporous adsorption resin.Method Two kinds of macroporous adsorption resin,the concentration and pH of the sample solution,the concentration and volume of eluent were optimized by using the contents of total saponins and flavonoids as the evaluating index.The reproducibility and service time of the resin were also studied.Results D101 macroporous adsorption resin was chosen as the desired resin.The optimal parameters of purification process were as follows:original concentration of the sample solution was 1.5 g·mL-1,in which the contents of total saponins and flavonoids were 6.52 mg·mL-1 and 4.81 mg·mL-1,respectively.The pH value of the sample solution was 4.0～5.0.The sample was eluted consecutively by water(8 BV),30% ethanol(4 BV) and 70% ethanol(4 BV) with the flow rate of 2 BV·h-1,and then the part eluted by 70% ethanol was gathered.Conclusion The optimal purification process was stable and feasible.