Simultaneous Separation and Purification Technology of Total Flavonoids and Total Saponins from Llex pubescens by AB-8 Macroporous Resin

Objective: To discuss purification technology conditions of total flavonoids and total saponins from Llex pubescens by macroporous resin.Method:With absorption rate and transfer rate of total flavonoids and total saponins as indexes,investigated concentration of sample liquid,pH,ratio of diameter to height of macroporous resin column,ratio of macroporous resin to crude drug,concentration of eluted ethanol and other factors.Result: Optimum purification conditions were as follows:concentration of sample liquid was 0.4 g·mL-1,pH of sample liquid was 4.5,ratio of crude drug to macroporous resin was 1∶8,ratio of diameter to height of macroporous resin column was 1∶15,total saponins were eluted by 12 BV water,10 BV 30% ethanol and 10 BV 70% ethanol respectively.Conclusion: Macroporous resin could be used to purify total flavonoids and total saponins from L.pubescens,the content of total flavonoid and total saponins reached more than 50%.