Cloning and analysis of the full-length cDNA of China reindeer ghrelin

In this study using the technology of rapid amplification of cDNA ends(RACE),3′RACE and 5′RACE were amplified on the basis of the known partial reindeer ghrelin cDNA sequence from our team.The results indicated that the sequences of 3′and 5′cDNA end were amplified successfully,and obtained the 604 bp full-length sequence of reindeer ghrelin cDNA,including a 5′untranslated region(UTR)of 57 bp,a open reading frame(ORF)of 405 bp,3′UTR of 128 bp and ploy(A)14.The ORF of 405 bp encodes a preproghrelin of 134 amino acids.The predicted preproghrelin was composed of a N-terminal signal peptide of 41 amino acid residues,a 27 residues mature ghrelin peptide and a 66 residues C-terminal peptide.The sequence alignment showed that the cDNA of reindeer ghrelin has homology of 92.0%,90.8% and 89.5% with goat,sheep and cattle respectively,69.5%and 65.2% with pig and human,only 33.8% with chicken.The results indicate that the structure of ghrelin has a obvious varietal speciality.The obtaining of full-length reindeer ghrelin lay the foundation of further studying the function of physiology.