The cDNA Cloning and Sequencing Analysis of Mongolia Sheep Ghrelin

In order to amplify cDNA of Mongolia sheep ghrelin,the differential primers were designed according to the GenBank sequence.Total RNA was extracted from the abomasums of Mongolia sheep and the cDNA encoding Mongolia sheep ghrelin was obtained by the reverse transcription PCR(RT-PCR).The purified RT-PCR product was cloned into pTZ19 vector.The results of restriction endonuclease pattern analysis of recombinant plasmid and DNA sequencing demonstrated that the Mongolia sheep ghrelin cDNA was part of ghrelin,because blasted sequencing result with published sheep ghrelin in NCBI web,only one base was different in 205 bases.The difference didn't influence polypeptide sequence after translation.The cDNA contained a ORF of 168 bases which encoded a 56 amino acid prepropeptide.The research to Mongolia sheep ghrelin will help us to open out its physiological and pathological function,and it will make far-reaching sense in preventing and curing some ruminant diseases.