cDNA Cloning of Reindeer Ghrelin

Ghrelin,an endogenous ligand for the growth hormone secretagogue receptor(GHS-R),is a kind of multi-peptide of 28 amino acid residues and can facilitate to release of GH.Total RNA was extracted from the abomasus tissue of reindeer and 300 bp gene fragment was obtained by RT-PCR with the primers which had been designed according to the published reindeer ghrelin gene sequence and synthesized.After being cloned into pBlueselect T vector,the PCR segment was determined as ghrelin cDNA by restriction endonuclease pattern analysis of recombinant plasmid and DNA sequencing.