Study on RAPD Reaction System for Gene Difference of the Cold and Warm Type of Wheat

In this research,with the same PCR program:94?℃/5 min→94?℃/45?s,38?℃/1 min,72?℃/1.5?min,45 cycle→72?℃/10 min→4?℃ forever,the effects of the compositions of RAPD-PCR reaction system on the gene difference of the cold and warm type of wheat were studied.An optimalzed RAPD system was set up: total volume was 25 μl,including DNA 20 ng template DNA,10×PCR buffer at 2.5?μl,dNTPs at 0.2 mmol/L,Taq DNA polymerase at 1?U,Mg~(2+) at 2.0 mmol/L,ddH_2O 12?μl and 10 ng random primers.