2003•Ganhanqu ziyuan yu huanjingRequires access
Optimal of RAPD amplification on Stipa L.
Mengli Zhao
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Abstract
CTAB method was used to extract genome DNA from plant material of Stipa L.Through optimal analysis of RAPD,a PCR amplification program was determined.When the amplification was performed as,94℃ pre-denaturation for 3min,45cycles of 94℃ for 1min,37℃ for 1min,72℃ for 1.5min,and 5min 72℃ extension,the 25μL reaction mixture contained 80ng DNA sample,0.2μL(10mmol/L) of each dNTP,2.0μL(10mmol/L) Mg 2+ ,0.5μL(100pmol/μL) primer,2.5X taq polymerase buffer,and 1 unit Taq polymerase(Sangon).