Genomic DNA extraction and optimization of RAPD-PCR conditions of Ginseng

Investigated the method of genomic DNA extraction and optimal RAPD-PCR condition.The results showed that the DNA were suitable for RAPD-PCR analysis extracted by CTAB method with some variant;the optimal RAPD-PCR contitions is as follow: in total 25 μL volumes of PCR reaction,containing 20 ng of DNA,200 μmol/L of dNTPs,1.5 mmol/L of Mg2+,2.5 μL of 10×buffer,0.4 μmol/L of primer,1.0 U of Taq DNA polymerase.The PCR program is:after predenaturing at 94 ℃ for 5 min,followed by 40 cycles of denatureing at 94 ℃ for 45 s,annealing at 40 ℃ for 1 min,extension at 72 ℃ for 1min,and final extension at 72 ℃ for 5 min.