Study on RAPD Reaction System for Dominant Multi-ovary Gene in Wheat

In this research, with the same PCR program: 94℃/5 min→94℃/1 min, 36℃/1 min,72℃/2 min,45 cycle→72℃/10 min→4℃ forever, the effects of compositions of RAPD-PCR reaction system for dominant multi-ovary gene in wheat were studied. An optimalized RAPD system was set up: the totle voume is 25 μl, (including) DNA 50 ng template DNA,10×PCR buffer at 2.5 μl, dNTPs at 0.2 mmol/L, Taq DNA (polymerase) at 1 u, Mg~(2+) at 2.0 mmol/L, ddH_2O 12.17 μl, and 10 ng random primers.