Distribution and antimicrobial resistance analysis of 151 strains isolated from blood

Objective To investigate the distribution and drug resistance of blood culture isolated bacteria in our hospital.Methods 1178 strains blood sample were collected in our hospital from June 2012 to December 2013.Blood sample were cultivated by the Bact/Alert-3D automatic blood culture system.The positive strains were identified by VITEK-C0MPACT2 automatic bacterial identification instrument.The drug sensitivity test were detected by K-B method and VITEK-COMPACT2 quantification MIC drug sensitivity analysis system.All data were analyzed by WHONET 5.4 software.Results 151 strains of bacteria were isolated from 1178 blood samples,and the isolated rate was 12.8%.In 151 strains of bacteria,the gramnegative bacteria was 97 strains(64.2%),gram-positive bacteria was 49 strains(32.5%),and fungi was 5strains(3.3%).In 97 strains of gram-negative bacteria,isolated rates of top three were Escherichia coli(39strains,25.8%),Klebsiella pneumoniae(25 strains,16.6%) and Brucella melitensis(15 strains,9.9%).In 49 strains of gram-positive bacteria,isolated rates of top two were Staphylococcus aureus(24 strains,15.9%) and Staphylococcus epidermidis(8 strains,5.3%).The mostly of fungi were Candida albicans(4 strains,2.6%).In39 strains of Escherichia coli and 25 strains Klebsiella pneumoniae,isolated rate of extended spectrum betalactamases were 46.2%(18/39) and 32.0%(8/25),respectively.In 24 strains of Staphylococcus aureus,isolated rate of methicillin-resistant Staphylococcus aureus was 50.0%(12/24).The resistant rates of Escherichia coli and Klebsiella pneumoniae to gentamicin,levofloxacin and ciprofloxacin were over 40.0%,and resistant rates of Staphylococcus aureus to gentamicin,piperacillin/tazobactam,cefoxitin and erythromycin were over 41.7%.Conclusion The gram-negative bacteria are majority in the blood culture isolated bacteria.The mroe herdsman in our region result in the much more Brucella melitensis.The drug resistance is serious,clinicians should be based on the identification of bacteria and drug sensitivity test to select sensitive drugs.