Extraction and Comparative Analysis of Berberine Hydrochloride Content from Cortex Phellodendri Chinense and Cortex Phellodendri Amurensis

Berberine hydrochloride was extracted from Cortex Phellodendri Chinense and Cortex Phellodendri amurensis, and the content of berberine hydrochloride was analyzed and compared. Extraction of berberine hydrochloride from Cortex Phellodendri Chinense and Cortex Phellodendri amurensis by ultrasonic method was carried out, and extraction conditions such as extraction reagent concentration, extraction time, extraction temperature and liquid ratio were optimized. The content of berberine hydrochloride in Cortex Phellodendri Chinense and Cortex Phellodendri amurensis was determined by HPLC, and the mobile phase was methanol-acetonitrile-phosphoric acid (0.01 mol/L) (10:28:62,V/V), the flow rate was 1 mL/min, and the detection wavelength was 350 nm. The sample input is 10μL. The linear range is 2.20 mm g/mL ~ 13.20 mm g/mL, the standard deviation (RSD) is less than 2.70 %, and the standard recovery rate is 93.21 ~ 99.38 %. The contents of berberine hydrochloride from Cortex Phellodendri Chinense and Cortex Phellodendri amurensis were 57.80mg/g and 48.20 mg/g respectively. The content of berberine hydrochloride in Cortex Phellodendri Chinense was higher than that in Cortex Phellodendri amurensis.