Determination of Alkaloids in Phellodendri Amurensis Cortex by HPLC

Objective: HPLC was used to measure the content of berberine,jatrorrhizine and palmatine in Phellodendri Amurensis Cortex from ten different areas of Heilongjiang Province simultaneously.Method: A Kromasil C 18 column(4.6 mm × 250 mm,5 μm) was used with the mobile phase consisting of acetonitril-0.1% phosphonic acid(25∶ 75,0.10 g sodium lauryl sulfate was added per 100 mL).The flow rate was 1 mL.min-1;the detecting wavelength was set at 345 nm,and the column temperature was maintained at 30 ℃.Result: The calibration curves of berberine hydrochloride,jatrorrhizine hydrochloride and palmatine hydrochloride were linear within the ranges of 12.08-144.96 mg.L-1(r = 0.999 3),1.60-25.60 mg.L-1(r = 0.999 3),7.26-87.12 mg.L-1(r = 0.999 3) respectively.The average recoveries(n = 9) were 102.51%,103.47%,102.89%,respectively.Conclusion: This method is proved to be convenient,reliable and accurate,it can be used in content determination of berberine,jatrorrhizine,palmatine in Phellodendri Amurensis Cortex.There are significant differences in the Alkaloid content in Phellodendri Amurensis Cortex from different areas.