The mature embryo of Ginkgo biloba L. in vitro culture and its cytohistological studies
Yuan-li Wu, Xuecheng Yan
Abstract
Yuan-li Wu, Xuecheng Yan
Abstract
Callus derived from completed embryos of Ginkgo biloba L. were light green and crisp. And after subculture, some callus changed from crispy to compact. In MS medium supplemented with 2mg/L BA and 0.5mg/L NAA, somatic embryos were obtained from the compact callus. Embryoids originated from embryonic cell at the edge of callus, and embryoids at whatever stage (globular or heart) exhibited a slow growth, which delayed transfer onto subculture medium.
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Callus derived from completed embryos of Ginkgo biloba L. were light green and crisp. And after subculture, some callus changed from crispy to compact. In MS medium supplemented with 2mg/L BA and 0.5mg/L NAA, somatic embryos were obtained from the compact callus. Embryoids originated from embryonic cell at the edge of callus, and embryoids at whatever stage (globular or heart) exhibited a slow growth, which delayed transfer onto subculture medium.
Key concepts: Callus, Subculture (biology), Somatic embryogenesis, Ginkgo biloba, Embryo, Botany, Somatic cell, Biology