Investigation of Extraction Process and Antioxidation of Total Flavonoids from Pilea aquarum

Objective: To determine the content of total flavonoids from Pilea aquarum and evaluate its antioxidative activity. Method: With the content of total flavonoids as an indicator, based on single factor experiments,response surface methodology was adopted to optimize extraction process by taking liquid-solid ratio,extraction temperature and time as factors. The content of total flavonoids in P. aquarum from different places were determined,then antioxidation of total flavonoids were evaluated against DPPH and ABTS free radical. Result:Optimum coditions were as follows: liquid-solid ratio of 1∶ 19,extraction temperature at 71 ℃ and extraction time of 126 min. Yield of total flavonoids was as high as 7. 08 mg·g- 1under these conditions. The content of total flavonoids in P. aquarumfrom different places was from 4. 35 to 7. 10 mg·g- 1with obvious regional differences.Scavenging capacity of total flavonoids were as same as vitamin C against DPPH and ABTS free radical.Conclusion: There is obvious difference of total flavonoids content in P. aquarum from different places,and total flavonoids has good antioxidative activity in a dose-dependent manner.