Study on extraction technology and antioxidant property of total flavonoids from cassia

The objective of this study was to optimize extraction technology of total flavonoids from cassia with ethanol extraction method.Based on single-factor test,the orthogonal test was carried out.The results showed that the optimal extraction conditions of total flavonoids were ethanol concentration 60%,extraction temperature 60℃,the ratio of solid to liquid 1:25,and extraction time 1.5h.Under these conditions,the extraction yield of total flavonoids form cassia was 79.82mg/g.DPPH radical scavenging experiment showed that cassia flavonoids possessed certain antioxidant property.Antioxidant property enhanced with flavonoids concentration increased.DPPH radical scavenging rate was more than 90% when flavonoids concentration was up to 0.4mg/mL.The IC50 result showed that the antioxidant property of the cassia flavonoids was lower than VC and BHT.