2011•FigshareOpen access
MCF-7 cells were treated with PMA, TSA and DesCoA (10 nM) in the indicated combinations for four hours prior to stimulation with insulin for 10 minutes
Kaiser, Christina, James, Stephen R
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Abstract
Copyright information:Taken from "Acetylation of insulin receptor substrate-1 is permissive for tyrosine phosphorylation"BMC Biology 2004;2():23-23.Published online 2 Nov 2004PMCID:PMC529456.Copyright © 2004 Kaiser and James; licensee BioMed Central Ltd. IRS-1 was immunoprecipitated and western blotted for the presence of IRS-1 itself, HDAC2 and phosphotyrosine. The graph shows average data for phosphotyrosine from three independent experiments (± SEM) normalised to the response to insulin alone.