MCF-7 cells were transfected with short inhibitory RNA oligonucleotides (80 and 160 pmol as indicated) for four hours and cultured a further 48 hours prior to treatment with PMA (10 ng/ml for 4 hours) and stimulation with insulin (100 nM) for 10 minutes

Copyright information:Taken from "Acetylation of insulin receptor substrate-1 is permissive for tyrosine phosphorylation"BMC Biology 2004;2():23-23.Published online 2 Nov 2004PMCID:PMC529456.Copyright © 2004 Kaiser and James; licensee BioMed Central Ltd. IRS-1 was immunoprecipitated and western blotted for the presence of HDAC2, phosphotyrosine, acetyl lysine and IRS-1 itself. The graphs show average data (± SEM) for 3–5 independent experiments, normalised to the insulin response alone. P-Y: phosphotyrosine.