MCF-7 cells were treated with IGF-1 (10 ng/ml) for the indicated times, PMA (10 ng/ml) for 4 or 6 hours as indicated or TSA (150 ng/ml) for 4 hours prior to lysis and immunoprecipitation of IRS-1 Western blots were probed for the presence of acetyl lysine

Copyright information:Taken from "Acetylation of insulin receptor substrate-1 is permissive for tyrosine phosphorylation"BMC Biology 2004;2():23-23.Published online 2 Nov 2004PMCID:PMC529456.Copyright © 2004 Kaiser and James; licensee BioMed Central Ltd. Densitometric analysis of acetylated IRS-1 was performed and is displayed in the histogram. This experiment has been repeated one other time although the effects of TSA on acetylation of IRS-1 have been observed in many other experiments.