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MCF-7 cells were treated with PMA and/or TSA for 4 hours (concentrations as in Figure 3) prior to stimulation with 100 nM insulin for 10 minutes
Kaiser, Christina, James, Stephen R
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Abstract
Copyright information:Taken from "Acetylation of insulin receptor substrate-1 is permissive for tyrosine phosphorylation"BMC Biology 2004;2():23-23.Published online 2 Nov 2004PMCID:PMC529456.Copyright © 2004 Kaiser and James; licensee BioMed Central Ltd. IRS-1 was immunoprecipitated and blotted for the presence of phosphotyrosine. The graph summarises results from 3 experiments (± SEM) normalised to the response to insulin stimulation alone.